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. 2006 Jun 27;361(2):243–256. doi: 10.1016/j.jmb.2006.06.021

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Copyright © 2006 Elsevier Ltd. All rights reserved.

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Supplementary Figure S1 — Kinetic analysis of cleavage at the uridylate in RNA R16.4 by Nsp15. Shown are the results from the three independent experiments in which the parameters were determined. Each experiment consisted of nine RNA concentrations analyzed over five time points. The mean K_m and V_max values derived from these experiments are summarized in Fig. 1B.