Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Jun 27;361(2):243–256. doi: 10.1016/j.jmb.2006.06.021

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006 Elsevier Ltd. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Examinations of Nsp15 interaction with RNA analogs. (a) A real time endoribonuclease assay for Nsp15. The sequence of the quenched fluorescent substrate named rU (IDT, Coralville, IA) is shown, with fluorophores FAM and tetramethylrhodamine. The only ribonucleotide in this construct, uridylate, is in a larger font and underlined. A second version in which the uridylate was replaced with ribocytidine (rC) is used as a control. The fluorescent outputs from the two substrates were determined in the presence and in the absence of Mn²⁺, as indicated to the right of the graph. (b) A graph demonstrating inhibition of fluorogenic rU substrate turnover in the presence of increasing concentration of unlabeled rU (no inhibitor), a 16 nt DNA(D16.4), or R16.4 analogs (PT16 and N3meU). The equation used for the curve fit is in the box where the effects of various treatments are summarized: m2 denotes the apparent K_m. (c) Image of an SDS/polyacrylamide gel where kinased RNAs (the identities of which are indicated above each lane) were crosslinked at 1200 μJ for 3 min to 1 μg of Nsp15. BSA was present at the same concentration. The letter ϕ is used to indicate that no protein was added in that reaction.