Table 2.
siRNAs used for gene silencing analysis.
| siRNA IDa | Gene symbol | Full gene target name | Ref. no.b | siRNA sequence 5′ → 3′c |
|---|---|---|---|---|
| AM4623 | Cy3-GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Commercial positive control, sequence not available | |
| #4390843 | NEG Control #1 | Commercial negative control, sequence not available | ||
| #4390849 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Commercial positive control, sequence not available | |
| s25665 | PABP | Poly(A)-binding protein, cytoplasmic 1 | #1 | CCUAAAUGAUCGCAAAGUAtt |
| UACUUUGCGAUCAUUUAGGag | ||||
| s25664 | PABP | Poly(A)-binding protein, cytoplasmic 1 | #2 | AUCCAUUGAUAAUAAAGCAtt |
| UGCUUUAUUAUCAAUGGAUtt | ||||
| s20563 | hnRNP Q (SYNCRIP) | Synaptotagmin binding, cytoplasmic RNA interacting protein | #1 | GAACGAGUGAAGAAGUUAAtt |
| UUAACUUCUUCACUCGUUCca | ||||
| s20564 | hnRNP Q (SYNCRIP) | Synaptotagmin binding, cytoplasmic RNA interacting protein | #2 | CAAUAGAGGUUAUGCGUUUtt |
| AAACGCAUAACCUCUAUUGag | ||||
| s4767 | EPRS | Glutamyl-prolyl-tRNA synthetase | #1 | CGAUGUCAGCAUUUCCGUUtt |
| AACGGAAAUGCUGACAUCGtc | ||||
| s4768 | EPRS | Glutamyl-prolyl-tRNA synthetase | #2 | CGUUAGAGCUGAUUUACGAtt |
| UCGUAAAUCAGCUCUAACGcg |
a
ID provided by the manufacturer (Ambion).
b
Arbitrary reference numbers used in this study.
c
Sense and antisense sequences are given in upper and lower rows, respectively. All the siRNAs used are synthetic duplexes of 21 nt with 2 nt-protruding 3′ ends (in lower case).