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. 2011 Jun 11;93(1):18–22. doi: 10.1016/j.rvsc.2011.05.009

Table 1.

PCR primers and conditions used in this study.

Primer Sequence (5′–3′) Target gene PCR product Reference strains Reference PCR Conditions
Stx1F CAGTTAATGTGGTGGCGAAGG stx1 348 bp C2103;
ED 669		 Vidal et al. (2005) 35× 90 s at 94 °C
90 s at 60 °C
90 s at 72 °C
Stx1R CACCAGACAATGTAACCGCTG
Stx2F ATCCTATTCCCGGGAGTTTACG stx2 584 bp C210–03 Vidal et al. (2005) 35× 90 s at 94 °C
90 s at 60 °C
90 s at 72 °C
Stx2R GCGTCATCGTATACACAGGAGC
EaeF TCAATGCAGTTCCGTTATCAGTT eae 482 bp C210–03;
ED 669		 Vidal et al. (2005) 35× 90 s at 94 °C
90 s at 60 °C
90 s at 72 °C
EaeR GTAAAGTCCGTTACCCCAACCTG
LtF GCACACGGAGCTCCTCAGTC ltII 218 bp H10407 Vidal et al. (2005) 35× 90 s at 94 °C
90 s at 60 °C
90 s at 72 °C
LtR TCCTTCATCCTTTCAATGGCTTT
StF AAAGGAGAGCTTCGTCACATTTT stII 129 bp EA-11 Vidal et al. (2005) 35× 90 s at 94 °C
90 s at 60 °C
90 s at 72 °C
StR AATGTCCGTCTTGCGTTAGGAC
Cnf1F GGGGGAAGTACAGAAGAATTA cnf1 1111 bp EF-176 Toth et al. (2003) 30× 60 s at 94 °C
60 s at 55 °C
60 s at 72 °C
Cnf1R TTGCCGTCCACTCTCTCACCAGT
Cnf2F TATCATACGGCAGGAGGAAGCACC cnf2 1240 bp EF-147 Toth et al. (2003) 30× 60 s at 94 °C
60 s at 55 °C
60 s at 72 °C
Cnf2R GTCACAATAGACAATAATTTTCCG
Cdt1F CAATAGTCGCCCACAGGA cdt-I 411 bp EF-133 Toth et al. (2003) 30× 60 s at 94 °C
60 s at 55 °C
60 s at 72 °C
Cdt1R ATAATCAAGAACACCACCAC
Cdt2F GAAAATAAATGGAATATAAATGTCCG cdt-II 556 bp 9142–88 Toth et al. (2003) 30× 60 s at 94 °C
60 s at 55 °C
60 s at 72 °C
Cdt2R TTTGTGTTGCCGCCGCTGGTGAAA
Cdt3F GAAAATAAATGGAATATAAATGTCCG cdt-III 555 bp EF-147 Toth et al. (2003) 30× 60 s at 94 °C
60 s at 55 °C
60 s at 72 °C
Cdt3R TTTGTGTCGGTGCAGCAGGGAAAA
Cdt4F CCTGATGGTTCAGGAGGCTGGTTC cdt-IV 350 bp E253 Toth et al. (2003) 30× 60 s at 94 °C
60 s at 55 °C
60 s at 72 °C
Cdt4R TTGCTCCAGAATCTATACCT
F17aF GCTGGAAGGGTGCAATACGCCTG f17a 321 bp 25KH9 Bertin et al. (1996) 25× 120 s at 94 °C
60 s at 55 °C
60 s at 72 °C
F17aR ATTCGTAACCCGCTCTCGTCC
F17bF CAACTAACGGGATGTACAGTTTC f17b 323 bp S5 Bertin et al. (1996) 25× 120 s at 94 °C
60 s at 55 °C
60 s at 72 °C
F17bR ATTCGTAACCCGCTCTCGTCC
F17cF GCAGGAACCGCTCCCTTGGC f17c 416 bp 31A Bertin et al. (1996) 25× 120 s at 94 °C
60 s at 55 °C
60 s at 72 °C
F17cR CAACTAACGGGATGTACAGTTTC
F17dF GATAGTCATAACCTTAATATTGCA f17d 239 bp 111KH86 Bertin et al. (1996) 25× 120 s at 94 °C
60 s at 55 °C
60 s at 72 °C
F17dR CAACTAACGGGATGTACAGTTTC