Table 3.
Test | Master bank | Work bank | Cell at the limit |
---|---|---|---|
Electron microscopy | + | − | + |
Reverse transcriptasea | + | − | + |
In vitro cell inoculation | + | − | + |
In vivo animal inoculation | + | − | + |
Antibody production testsb | + | − | − |
Infectivityc | + | − | + |
Other virus specific testsd | + | − | + |
Not necessary if positive by retrovirus infectivity test.
e.g., MAP, RAP, HAP—Usually applicable for rodent cell lines.
For retroviruses and other endogenous viruses.
Tests for cell lines derived from human, non-human primate or other cell lines as appropriate.