Fig. 3.

METTL3-induced cell survival depends on glycolytic metabolism. (a) Cell proliferation assay was performed by CCK8 assay in HCT116 WT or METTL3-KO cells, n = 6, nonparametric Mann–Whitney test. (b) Colony formation assay was performed in HCT116 WT or METTL3-KO cells, n = 3, nonparametric Mann–Whitney test. (c) Representative images of tumors in nude mice bearing HCT116 WT or METTL3-KO cells, n = 8. (d-e) Tumor volumes(d) and tumor weights (e) were measured in mice bearing HCT116 WT or METTL3-KO cells, n = 8, nonparametric Mann–Whitney test. (f) Cell proliferation of DLD-1 cells was measured by CCK8 assay after transfection of pcDNA3.1, pcDNA3.1-METTL3 and pcDNA3.1-METTL3-mut (METTL3 with MTase domain deletion), n = 6, nonparametric Mann–Whitney test. (g) Colony formation assay was performed in DLD1 cells after transfection with pcDNA3.1, pcDNA3.1-METTL3 and pcDNA3.1-METTL3-mut; n = 3, nonparametric Mann–Whitney test. (h-j) Representative images of tumors (h), statistical analysis of tumor volumes (i) and tumor weights (j) in nude mice bearing DLD1 cells in different groups (n = 8, nonparametric Mann–Whitney test). Fig 3h, Fig. 3m and Figure S6j shared experimental controls and METLL3-overexpression group. (k-l) Cell proliferation (k) and colony formation assay (l) were performed in DLD1 cells after transfection with control plasmids, METTL3 overexpression plasmids, and subsequently with 5 mM 2-DG (2-Deoxyglucose) treatment; nonparametric Mann–Whitney test. (m-o) Representative images of tumors (m), statistical analysis of tumor volumes (n) and tumor weights (o) in nude mice bearing DLD1 cells in different groups (n = 8, nonparametric Mann–Whitney test). Fig. 3h, Fig. 3m and Figure S6j shared experimental controls and METTL3-overexpression group. (WT, wild type; METTL3-KO, METTL3-knockout)