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. 2020 Apr 3;18:57. doi: 10.1186/s12951-020-00612-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — a The BTZ-NP uptake by the 4T1 cells; b The viability curves of 4T1 cells after incubation with BTZ-NP or free BTZ for 24 h, measured by MTT method; c The 4T1 cell apoptosis detected by TEM (the scale bar represents 200 nm); d The live/dead assay of 4T1 cells exposed to PBS, BTZ-NP or free BTZ, measured by Calcein/PI; e The caspase 3 expression in 4T1 cells exposed to PBS, BTZ-NP or free BTZ, measured by the GreenNuc kit