Fig. 2.

Inhibitory effect of vivo-MOs in RD cells. Various concentrations of vivo-MOs were applied to RD cells one hour post infection and (A) viral induced CPE (20 × objective) were observed 24 hpi. The total infectious particles or total viral proteins were harvested 24 hpi and evaluated by (B) plaque assay, (C) quantitative TaqMan real-time PCR, and (D) western blot analysis, respectively. EV-71 viral capsid protein was detected by mouse anti-EV-71 monoclonal antibody (Millipore) and cellular β-actin was detected using mouse anti-β-actin monoclonal antibody (Sigma). The data presented were obtained from at least two independent biological replicates.