Fig. 1.

(A) 3D HPLC-profile of MeOH (50%) soluble portion of Flos Lonicerae extract; the MeOH soluble portion of Flos Lonicerae extract was filtered through a 0.45 μm membrane filter and the resulting filtrate was subjected for HPLC analysis. The analyses were performed using a Waters 2695 Alliance HPLC system (Waters Corp., Milford, MA, USA), consisting of a quaternary pump solvent management system, an on-line degasser, and an autosampler. The raw data were detected by 2998 PDA, acquired, and processed with Empower™ software. A Hypersil ODS C18 column (250 × 4.6 mm, 5 μm) (Thermo Scientific, Waltham, MA, USA) was applied for all analyses. The mobile phase was composed of A (acetonitrile) and solvent B (0.4% aqueous phosphoric acid, V/V) with a linear gradient elution: 0–20 min, 10–14% A; 20–50 min, 14–30% A; 50–55 min, 30–10% A; 55–60 min, 10% A. The mobile phase flow rate was 1 ml min⁻¹, the column temperature was controlled at 30 °C and sample injection volume was 10 μl. (B) UPLC–MS/MS profile of Flos Lonicerae extract to determination phenolic acids simultaneous (unpublished).