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. 2020 Mar 27;10:348. doi: 10.3389/fonc.2020.00348

Figure 5.

Figure 5

Inhibition of autophagy will aggravate cell death of lung cancer cells induced by NiPT A549 and NCI-H1299 cells were treated with or without 5 μM NiPT in the presence of 100 nM bafilomycin, 3MA, or E64D, respectively. (A) Cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) assay. Data from three repeats. Mean+SD (n = 3). (B) Cells were stained with AnnexinV/PI and examined with flow cytometry. (C) Cells were stained with AnnexinV/PI and imaged under a fluorescent microscopy.