Fig. 5.

RRV lacking E2 N-linked glycans retain ability to replicatein vivo. Quadriceps muscle (A) and ankle joints (B) from mice infected with RRV WT (solid circles), E2 N200Q (open circles), E2 N262Q (solid squares) or E2 DM (open square) were assayed to determine viral titer at various times post infection. Viral titer was determined by plaque assay on BHK-21 cells. *** p < 0.005 ** p < 0.01 by one-way ANOVA. (C) Quantification of total numbers of RRV RNA genomes per µg of RNA from the quadriceps muscles of mice infected with either RRV WT (solid circles) or E2 DM (open squares). ** p < 0.01 by Student's t-test.