Fig. 6.

The RRV E2 glycan mutants and WT RRV induce equivalent inflammation within skeletal muscle. Leukocytes were isolated from the quadriceps muscle of mice infected with RRV WT (solid circles), E2 N200Q (open circles), E2 N262Q (solid squares) or E2 DM (open squares) mice at 10 dpi. Cells were characterized and quantified by flow cytometry using the markers indicated on Y-axis. Total numbers of leukocytes, inflammatory macrophages and other inflammatory cell types are shown. Each data point represents a single animal, and data presented in the figure are combined from three independent experiments.