Fig. 1.

Hematoxylin and eosin stained photomicrographs (a-d), 14–3-3 β/α, 14–3-3 ζ/δ, 14–3-3 γ, and 14–3-3 ε protein spots (e and f), and graph of each spots intensity (g-j) from neonatal cerebral cortices, of vehicle- (a, c and e) and glutamate treated (b, d and f) neonatal rats. Among 6 layers of cerebral cortex, external pyramidal layer (III) was observed (a and b). Arrows indicate normal neurons with well-developed dendrites and typical pyramidal shape (c). Arrowheads indicate damaged neurons with shrunken dendrites and atypical round shape (d). Scale bar: 50 μm. Arrows and numbers indicate 14–3-3 proteins spots (e and f). (1) 14–3-3 β/α, (2, 3) 14–3-3 ζ/δ, (4) 14–3-3 γ, (5) 14–3-3 ε. Spot intensities were measured using PDQuest software. The spot intensities are reported as a ratio relative to control animals. Data (n = 4) are shown as mean ± S.E.M. * P < 0.05