Fig. 3.

Western blot analyses of primary CNC cells and ES cell specimen using the 4E9R antibody. Comparative immunoblot analysis of CNC cells (1) as well as ES cell-derived specimens at various differentiation stages: 2) undifferentiated ES cells, 3) 5d EBs; 4) 5＋2d; 5) 5＋4d; 6) 5＋7d; 7) 5＋9d; 8) 5＋11d; 9) 5＋14d; 10) 5＋18d; 11) 5＋21d; 12) was performed using 4E9R antibody. For protein integrity the reference protein Gapdh was used as loading control. E: embryonic day, undiff.: undifferentiated, d: day, EB: embryoid body.