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. 2020 Apr 2;8:47. doi: 10.1186/s40168-020-00826-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Comparison between the polar microbiomes and the Tara Oceans data. a Venn diagram showing the distribution of operational taxonomic units (OTUs) across the polar and the Tara miTags. The Tara miTags comprise 16S rRNA sequences extracted from 243 seawater metagenomes. Venn diagrams based on OTUs with more than 2, 10, 50 miTag numbers are shown. b The change of the taxonomic specificity along with the OTU abundances (indicated by the reads number of OTUs) included for comparison. c Establishment of a polar microbial reference gene catalog (PM-RGC) and comparison with the ocean microbial reference gene catalog (OM-RGC) established by the Tara Oceans study. PM-RGC specific gene orthologs are nonredundant genes that are present in the polar seawater metagenomes but were not detected in the OM-RGC. d BLASTp searching the NCBI-Nr database using the PM-RGC or OM-RGC specific gene catalog. Genes got hit in NCBI-Nr database are labeled as “known” while genes with no hit are labeled as “novel”