Figure 5.

Immunohistochemical staining of mouse kidneys. Mice were fed a control diet, high-fat diet (HFD) or HFD supplemented with lingonberry (HFD+LB) for 12 weeks. Paraffin embedded kidney sections were stained with (A) anti-F4/80 antibody to detect macrophages or (B) anti-MPO antibody to detect neutrophils in the inflamed areas. Arrows point to the infiltrated macrophages and neutrophils; the images were captured at 200X. Inset: Areas containing F4/80 or MPO positive cells were enlarged 2X (Scale bar = 100 μm).