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. 2020 Mar 27;7:80. doi: 10.3389/fmed.2020.00080

Table 1.

Primer sequences used for RT-qPCR.

Primer Sequence 5′-3′ Accession number Product length
Human
IL-6 F: ACTCACCTCTTCAGAACGAATTG XM_005249745.5 149 bp
R: CCATCTTTGGAAGGTTCAGGTTG
MCP-1 F: CCCAAAGAAGCTGTGATCTTCA NM_002982.4 186 bp
R: GTGTCTGGGGAAAGCTAGGG
TNF-α F: GAGGCCAAGCCCTGGTATG NM_000594.4 91 bp
R: CGGGCCGATTGATCTCAGC
β-Actin F: AGATCAAGATCATTGCTCCTCCT NM_001101.5 95 bp
R: GATCCACATCTGCTGGAAGG
Mouse
IL-6 F: GACTGATGCTGGTGACAACC NM_001314054.1 170 bp
R: GCCATTGCACAACTCTTTTC
MCP-1 F: AGGTCCCTGTCATGCTTCTG NM_011333.3 167 bp
R: GCTGCTGGTGATCCTCTTGT
TNF-α F: GTCCCCAAAGGGATGAGAAG NM_001278601.1 93 bp
R: GCTCCTCCACTTGGTGGTTT
NGAL F: ACGGACTACAACCAGTTCGC NM_008491.1 192 bp
R: AATGCATTGGTCGGTGGGG
KIM-1 F: TCCACACATGTACCAACATCAA XM_011248784.2 98 bp
R: GTCACAGTGCCATTCCAGTC
β-Actin F: GATCAAGATCATTGCTCCTCCT XM_030254057.1 183 bp
R: AGGGTGTAAAACGCAGCTCA