Table 2.
Detection of CSFV in semen from experimentally infected boars by virus isolation and ReTi-PCR
| Days post-infection | Boar A |
Boar B |
Boar C |
|||
|---|---|---|---|---|---|---|
| Virus isolation | ReTi-PCR | Virus isolation | ReTi-PCR | Virus isolation | ReTi-PCR | |
| 0 | − | − | − | − | − | − |
| 3 | − | − | − | + | − | − |
| 5 | +* | + | ||||
| 6 | − | − | − | − | ||
| 7 | + | + | ||||
| 8 | +* | − | +* | + | ||
| 9 | + | + | ||||
| 10 | +* | − | − | + | ||
| 11 | +* | + | ||||
| 12 | − | + | − | − | ||
| 13 | − | − | ||||
| 14 | − | − | − | − | ||
| 15 | − | + | ||||
| 16 | − | + | − | + | ||
| 17 | − | + | ||||
| 18 | − | − | − | + | ||
Only sampled on the indicated days post-infection. +: Positive result in the ReTi-PCR or virus isolation; +*: positive result in the virus isolation after one blind passage.