Table 3.
Detection of FMDV in semen from experimentally infected boars by virus isolation and ReTi-PCR
| Days post-infection | Boar 1 |
Boar 2 |
Boar 3 |
Boar 4 |
||||
|---|---|---|---|---|---|---|---|---|
| Virus isolation | ReTi-PCR | Virus isolation | ReTi-PCR | Virus isolation | ReTi-PCR | Virus isolation | ReTi-PCR | |
| 0 | − | − | − | − | − | − | − | − |
| 1 | ns | ns | − | + | ||||
| 2 | − | − | ns | ns | − | + | − | + |
| 3 | − | − | − | − | ||||
| 4 | − | − | − | − | ||||
| 5 | # | # | − | − | ||||
| 6 | − | − | − | + | ||||
| 7 | ns | ns | ||||||
| 9 | − | − | − | + | ||||
| 10 | # | # | − | + | − | − | ||
| 11 | # | # | −# | +# | ||||
Only sampled on the indicated days post-infection. +: Positive result in the ReTi-PCR or virus isolation; ns: no semen collected; #: boar killed because of severe clinical signs of FMD.