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SHRT-PCR equipment and principles: SHRT-PCR unit, UK-104MK IV (A), triple heat blocks (B), compact disc-type sample container (C), and schematic diagram of SHRT-PCR technique (D). The disc-type thin sample container is rotated over heat blocks at 3 different temperatures (for denaturing, annealing, and extension), resulting in rapid temperature changes in the samples.
