Table 3.

Reaction conditions for SHRT-PCR.

Step	Temperature	Duration	Cycles
Reaction conditions using original reaction mixture
cDNA synthesis	55 °C	3 min	Hold
Inactivation of ReverTra Ace	95 °C	1 min	Hold
Denaturing	95 °C	6 s each (total 18 s)	40 cycles
Annealing	58 °C
Extension	75 °C
Reaction conditions using RNA-Direct™ pre-mixture
Inactivation of antibody	90 °C	30 s	Hold
cDNA synthesis	55 °C	5 min	Hold
Pre-denature	90 °C	30 s	Hold
Denaturing	90 °C	Each 6 s (total 18 s)	40 cycles
Annealing	52 °C
Extension	70 °C