Table 3.

Intra-assay and inter-assay reproducibility of parainfluenza viruses standard curve by RT Real Time Qt-PCRs.

Standard dilution (plasmid copies/reaction)	Intra-test (%)			Inter-test (%)
	HPIV-1	HPIV-2	HPIV-3	HPIV-1	HPIV-2	HPIV-3
Coefficient of variability
102	0.29	2.90	2.32	1.00	1.15	3.88
103	0.96	0.11	1.71	0.42	0.44	4.04
104	0.79	0.55	2.46	0.39	1.25	0.27
105	0.77	1.33	1.89	1.11	0.59	1.56
	y = 34.9 − 2.6 log(x) R2 = 0.992	y = 37.9 − 2.6 log(x) R2 = 0.990	y = 38.4 − 2.9 log(x) R2 = 0.969	y = 40.3 − 3.5 log(x) R2 = 0.990	y = 42.3 − 3.2  log(x) R2 = 0.989	y = 43.1 − 4.0 log(x) R2 = 0.965

Equations of linear regression curves are indicated.