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. 2010 Jun 25;169(1):198–201. doi: 10.1016/j.jviromet.2010.06.007

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Copyright © 2010 Elsevier B.V. All rights reserved.

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Fig. 1 — Standard curve (plot of the Ct values against the different plasmid DNA concentrations) indicating the linearity and efficiency of the SYBR^® Green reactions for VP2 gene of CPV 2. The assays were linear over a 10⁷ dilution range with R² values (square of the correlation coefficient) of 0.998, the slope value of −3.412 and reaction efficiency of 96.4%.