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. 2002 Nov 12;32(3):243–259. doi: 10.1016/0165-2427(92)90049-V

Swine leukocyte antigen and macrophage marker expression on both African swine fever virus-infected and non-infected primary porcine macrophage cultures☆

Mercedes Gonzalez Juarrero a, Charles A Mebus b, Reyes Pan c, Yolanda Revilla c, Jose M Alonso c, Joan K Lunney d,+
PMCID: PMC7119579  PMID: 1632065

Abstract

Swine leukocyte antigens (SLA) and a macrophage specific marker were monitored on porcine macrophages cultured with or without macrophage colony stimulatory factor (M-CSF) and on cells infected with African swine fever virus (ASFV). SLA expression was maximal either in the total cell extract or on the cell surface at 3–4 days of culture; after 4 days these values began to decrease. Fluorescence analyses of immunostained macrophages cultured with or without M-CSF indicated a major upward shift in the number of SLA Class I molecules on individual macrophages whereas for SLA Class II both a novel expression of Class II and an upward shift in the number of molecules per cell were evident.

Infection of 3-day-old macrophage cultures with three different isolates of ASFV resulted in minor changes in surface expression of SLA Class I, SLA Class II, and macrophage markers. No differences in infection with ASFV was observed whether macrophages were SLA Class II positive or negative, nor was there blocking by anti-SLA Class I or Class II monoclonal antibodies of ASFV infection of cultured macrophages.

Abbreviations: APC, antigen presenting cell; ASF, African swine fever; ASFV, African swine fever virus; c.p.e., 50% cytopathic effect; DR-II, Dominican Republic II ASFV isolate; HAD50, 50% hemadsorption units; LPS, lipopolysaccharide; L60, Lisbon 60 ASFV isolate; mAb, monoclonal antibody; M-CSF, macrophage colony stimulatory factor; MHC, major histocompatibility complex; NHV, non-hemadsorbing ASFV isolate; PBMC, peripheral blood mononuclear cells; PBS, phosphate-buffered saline; SLA, swine leukocyte antigen

Footnotes

☆

This work was supported by the US-Spain Joint Committee for Scientific and Technological Cooperation, program No. G11.

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