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Comparison of the techniques used to detect RSV in infected MRC5 cells
| Dilutiona | Qualitative RT-PCR hybridisation-EIA | Culture | Real-time RT-PCR assay for RSV | |
|---|---|---|---|---|
| RSV A long | 10−5 | + | + | + |
| 10−6 | − | + | + | |
| RSV B1 | 10−4 | + | + | + |
| 10−5 | − | + | + | |
| 10−6 | − | − | − | |
| RSV A wt no. 1 | 10−4 | + | + | + |
| 10−5 | − | + | + | |
| 10−6 | − | − | + | |
| RSV A wt no. 2 | 10−5 | + | + | + |
| 10−6 | − | − | + | |
| RSV B wt | 10−5 | + | + | + |
| 10−6 | − | − | + |
The other tested dilutions were all positive (i.e. 10−3 or 10−4) or all negative (i.e. 10−7).
