Table 1.
Sequences of primers and probes used in this study
| Virus | Primer and probe name | Sequence (5′–3′) | Nucleotide position | Amplicon size (bp) |
|---|---|---|---|---|
| TGEV | TGENF TGENR | GCAGGTAAAGGTGATGTGACAA ACATTCAGCCAGTTGTGGGTAA | 27,637−27,756a | 120 |
| TGE-FAM | 6FAM-TGGCACTGCTGGGATTGGCAACGA-BHQ1 | 27,707–27,730 | ||
| PEDV | PEDNF PEDNR | CGCAAAGACTGAACCCACTAATTT TTGCCTCTGTTGTTACTTGGAGAT | 26,679−26,876b | 198 |
| PED-Cy5 | Cy5-TGTTGCCATTGCCACGACTCCTGC-BHQ3 | 26,819–26,842 | ||
a
The position of the primer is based on the Purdue strain of TGEV.
b
The position of the primer is based on the CV777 strain of PEDV.