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Detection the transcription of the plasmids by RT-PCR. Eukaryotic expression of the plasmids: pIRES-N/IL2, pIRES-N in Vero cells. Total RNA was extracted from Vero cells 36 h after transfection with pIRES-N/IL2, pIRES-N. The templates used for RT-PCR were as follows: lane 1, Marker III; lane 2, negative control; lane 3, plasmid pIRES-N/IL2; lane 4, plasmid pIRES-N.
