Fig. 1.
Growth curve of the continuous porcine myelomonocytic single cell clones 3D4/2 (♦), 3D4/21 (■) and 3D4/31 (▴) in 15% FBS/RPMI. Cells were seeded in six-well plates at approximately 2×105 cells/well in 15% FBS/RPMI, nonessential amino acids (1:100) and gentamycin (5 μg/ml). Three wells per clone were trypsinized at 22, 28, 48, 72, 96, 116 and 142 h post seeding, and the cells from each well were counted The obtained average cell count for each clone at each time point was plotted against the time.