Table 3.

Effect of culture media formulation on ASFV replication in continuous porcine monocyte/macrophage clones 3D4/2, 3D4/21 and 3D4/31 in comparison to standard cell culture systems

Medium/supplement	ASFV-Lisbon 61 (titer log 10)				ASFV-Lillie (titer log 10)
	Vero	3D4/2	3D4/21	3D4/31	PMBC	3D4/2	3D4/21	3D4/31
(1) RPMI/10% FBS	6.00	5.50	6.25	6.70	6.50	3.00	3.75	3.75
(2) RPMI/10% FBS 1:M PMA		5.80	5.80	6.50		3.00	4.00	2.75
(3) RPMI/10% FBS 1% DMSO		5.00	6.25	6.50		3.50	5.00	2.50
(4) SFM		4.75	3.75	6.00		3.50	3.50	2.75
(5) *RPMI/10%PorS	6.10*	4.50	4.60	4.65	5.10*	**	**	**

^*New virus stocks.

^**Cells in the 10⁻¹ virus dilution washed off. No IHC staining was detected in 10⁻² virus dilution.