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IBV nucleocapsid gene fragments. (a) Electrophoresis in 1.5% agarose gel. Amplified PCR products of the nucleocapsid gene fragments. (b) Electrophoresis in 1.5% agarose gel. The nucleocapsid gene fragments isolated from pQEN4IBV and pQEN2IBV (N4 fragment and N2 fragment, respectively) by the restriction with BamHI and HindIII. N4 fragment, 809 bp; N2 fragment, 398 bp. (c) Locations of overlapping N gene fragments of IBV encoding four (N4 fragment) and two (N2 fragment) antigen sites.
