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. 2006 May 3;135(2):292–296. doi: 10.1016/j.jviromet.2006.03.019

Fig. 2.

Fig. 2

SDS-PAGE on a 12.5% gel and Western blotting. (a) SDS-PAGE of bacterial lysates. Lane 1, lysate of E. coli cells in the absence recombinant plasmid; lane 2, MW marker; lane 3, lysate of E. coli cells with pQEN2IBV after IPTG expression; lane 4, lysate of E. coli cells with pQEN2IBV after IPTG expression. (b) SDS-PAGE of rNp2IBV and rNp4IBV mixture and purified and concentrated antigen of IBV. Lane 1, MW marker; lane 2, antigen of IBV; lane 3, mixture of recombinant proteins. (c) Western blot analysis. Lane 1, MW marker. The transferred mixture of rNp2IBV and rNp4IBV and proteins of IBV antigen were reacted with rNp2IBV-specific chicken serum (lanes 2 and 3, respectively), with rNp4IBV-specific chicken serum (lanes 4 and 5, respectively), with IBV-specific chicken serum (lanes 6 and 7, respectively), with normal chicken serum (lanes 8 and 9, respectively).