Table 1.
Specificity | pAb/mAb (clone) | Host of origin | Treatment | Dilution | Source |
CD3 | pAb | Human | Pronasea | 1/150 | Dako (Denmark) |
CD79α | mAb (HM57) | Human | Microwaveb | 1/25 | Dako (Denmark) |
L1 | mAb (MAC387) | Human | Pronase | 1/200 | Dako (Denmark) |
Lysozyme | pAb (A099) | Human | Trypsinc | 1/100 | Dako (Denmark) |
CD45RA | mAb (3C3/9) | Swine | No treatment | Undilutedd | INIA Lab |
SLA-DQ | mAb (BL2H5) | Swine | Pronase | Undilutedd | INIA Lab |
Incubation with 0.1% pronase for 10min at 37°C.
Incubation with citrate buffer (pH 6) for 20min at 100°C.
Incubation with 0.1% trypsin for 2h at 37°C.
Culture surnatant.