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. 2003 Jun 25;94(1):63–75. doi: 10.1016/S0165-2427(03)00079-5

Table 1.

Details of the primary antibodies used in the immunohistochemical study

Specificity pAb/mAb (clone) Host of origin Treatment Dilution Source

CD3 pAb Human Pronasea 1/150 Dako (Denmark)
CD79α mAb (HM57) Human Microwaveb 1/25 Dako (Denmark)
L1 mAb (MAC387) Human Pronase 1/200 Dako (Denmark)
Lysozyme pAb (A099) Human Trypsinc 1/100 Dako (Denmark)
CD45RA mAb (3C3/9) Swine No treatment Undilutedd INIA Lab
SLA-DQ mAb (BL2H5) Swine Pronase Undilutedd INIA Lab
a

Incubation with 0.1% pronase for 10min at 37°C.

b

Incubation with citrate buffer (pH 6) for 20min at 100°C.

c

Incubation with 0.1% trypsin for 2h at 37°C.

d

Culture surnatant.