Table 2.

Analytical sensitivity and limit of detection of the singular and triplex assays analyzed by standard curves using plasmid DNA or RNA as templates.

Virus	PCR Type	Plasmid DNA			RNA
		R2	E	LOD	R2	E	LOD
PRV	Singular	0.998	95.20%	5	N/A	N/A	N/A
	Triplex	0.992	100.80%	6	N/A	N/A	N/A
PPV	Singular	0.991	105.00%	3	N/A	N/A	N/A
	Triplex	0.996	97.30%	5	N/A	N/A	N/A
PCV-2	Singular	0.998	90.40%	1	N/A	N/A	N/A
	Triplex	0.991	93.70%	2	N/A	N/A	N/A
SVDV	Singular	0.998	108.70%	2	0.997	94.2%	5
	Triplex	0.994	98.10%	2	0.981	91.3%	5
VSV-IN	Singular	0.997	106.90%	6	0.992	102.7%	5
	Triplex	0.997	96.10%	7	0.996	94.9%	14
VSV-NJ	Singular	0.997	103.70%	4	0.995	92.5%	5
	Triplex	0.999	98.60%	8	0.996	96.9%	10
ASFV	Singular	0.992	107.20%	1	N/A	N/A	N/A
	Triplex	0.999	95.90%	3	N/A	N/A	N/A
CSFV	Singular	0.998	90.90%	3	0.999	110.2%	6
	Triplex	0.994	91.00%	4	0.994	92.2%	16
FMDV	Singular	0.998	100.30%	5	0.998	102.9%	5
	Triplex	0.998	98.40%	5	0.982	93.6%	5
SIV	Singular	0.999	98.4%	1	0.995	94.7%	N/A
	Triplex	0.998	105.3%	3	0.996	110.3%	N/A
PRRSV-NA	Singular	0.999	99.00%	3	0.997	99.2%	N/A
	Triplex	0.997	92.50%	5	0.995	107.6%	N/A
PRRSV-EU	Singular	1.000	99.90%	2	0.990	103.9%	N/A
	Triplex	0.998	105.30%	3	0.998	103.6%	N/A

R²: Correlation coefficient; E: PCR amplification efficiency (calculated by E = 10^−1/slope-1); LOD: Limit of detection (Copies/μL).