Table 4.
Comparison among the newly developed triplex SIV, PRRSV-NA and PRRSV-EU real time RT-PCR assay with existing real time RT-PCR assays using clinical samples.
| Clinical samples | Target | Newly developed real time RT-PCR |
KSU real time RT-PCR |
||||
|---|---|---|---|---|---|---|---|
| Triplex of SIV/PRRSV-NA/PRRSV-EU | SIV singular | PRRSV-NA singular | PRRSV-EU singular | PRRSV | SIV | ||
| 80 sera | SIV | 1 | 1 | 0 | 0 | NT | NT |
| PRRSV-NA | 43 | 0 | 43 | 0 | 44 | NT | |
| PRRSV-EU | 1 | 0 | 0 | 1 | |||
| SIV + PRRSV-NA | 5 | 5 | 5 | 0 | NT | NT | |
| 53 lung tissues | SIV | 28 | 28 | 0 | 0 | NT | 28 |
| PRRSV-NA | 3 | 0 | 3 | 0 | NT | NT | |
| PRRSV-EU | 0 | 0 | 0 | 0 | NT | NT | |
| SIV + PRRSV-NA | 5 | 5 | 5 | 0 | NT | NT | |
NT: Not tested.