Table 2.
Overall performance of nested (both previously published and designed in this study) and new singleplex PCR primer sets test in a dataset of 132 putatively CFPHV DNA extracts.
| Primer sets | Individual primer set performance |
Overall performance |
||
|---|---|---|---|---|
| Total positive PCR viral detections per individual primer set | Proportion of viral detection in total infected cases (n = 132) | Overall viral detection per PCR assay method | Proportion of viral detection per PCR assay method in total infected cases (n = 132) | |
| Nested primers | ||||
| Lu.et.(UL30) | 22 | 16.7% | Previously published Lu.et.(UL30) 22 |
16.7% |
| UL18 | 25 | 18.9% | ||
| UL22 | 37 | 28.0% | Combined nested UL18 + UL22 + UL27 44 |
33.3% |
| UL27 | 27 | 20.5% | ||
| Singleplex primers | ||||
| UL18 | 58 | 43.9% | Combined singleplex UL18 + UL22 + UL27 132 |
100% |
| UL22 | 93 | 70.5% | ||
| UL27 | 76 | 57.6% | ||