Table 1.
Amino acid variations in the VP2 protein of different CPV types
| Amino acid variations at residuea |
||||||||
|---|---|---|---|---|---|---|---|---|
| 87 | 101 | 297 | 300 | 305 | 375 | 426 | 555 | |
| 3045–3047b | 3087–3089b | 3675–3677b | 3684–3686b | 3699–3701b | 3909–3911b | 4062–4064b | 4449–4451b | |
| ATG (Met)c | ATT (Ile)c,d | TCT (Ser)c | GCT (Ala)c | GAT (Asp)c | AAT (Asn)c | AAT (Asn)c | GTA (Val)c | |
| TTG (Leu) | ACT (Thr)d | GCT (Ala) | GGT (Gly) | TAT (Tyr) | GAT (Asp) | GAT (Asp), GAA (Glu) | ATA (Ile) | |
| CPV-2 | Met | Ile | Ser | Ala | Asp | Asn | Asn | Val |
| CPV-2a | Leu | Thr | Ser | Gly | Tyr | Asp | Asn | Ile |
| CPV-2b | Leu | Thr | Ser | Gly | Tyr | Asp | Asp | Val |
| New CPV-2b | Leu | Thr | Ala | Gly | Tyr | Asp | Asp | Val |
| New CPV-2a | Leu | Thr | Ala | Gly | Tyr | Asp | Asn | Val |
| Asp-300 (CPV-2a/CPV-2b) | Leu | Thr | Ala | Asp | Tyr | Asp | Asn Asp | Val |
| CPV-2c | Leu | Thr | Ala | Gly | Tyr | Asp | Glu | Val |
a
Positions are referred to the amino acid and nucleotide sequences of strain CPV-b (accession no. M38245).
b
Nucleotide position.
c
Codon observed.
d
Codon affected by the SNP used to design the type-specific probes CPV2-Pb (type 2) and CPVv-Pb (variants).