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. 2007;386:3–55. doi: 10.1007/978-1-59745-430-8_1

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© Humana Press Inc. 2007

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Fig. 18 — One-step preparative reversed-phase (RP)-high-performance liquid chromatography (HPLC) purification of a recombinant protein from a whole cell lysate. Top, analytical RP-HPLC profile of crude sample mixture. Bottom: pooled fractions following preparative purification of recombinant protein Tm 1–99. Column, conditions of analytical RP-HPLC, preparative RP-HPLC, and fraction analysis described under Subheading 3.6.2. P denotes desired product. (Reproduced from ref. 84, with permission from Elsevier Science.)