Table 6.
Sequences of Two-dimensional Cation-Exchange/Reversed-Phase Chromatography Peptide Standards6
| Peptide Standard | Net Charge | Change in Carbon atom Content | Peptide Sequence | Mass | Change in Mass |
|---|---|---|---|---|---|
| 1a | +1 | 0 | Ac-Gly-Gly-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-amide | 814 | 0 |
| 1b | +1 | 1 | Ac-Gly-Gly-Ala-Gly-Gly-Leu-Gly-Leu-Gly-Lys-amide | 828 | 14 |
| 1c | +1 | 2 | Ac-Gly-Gly-Ala-Ala-Gly-Leu-Gly-Leu-Gly-Lys-amide | 842 | 28 |
| 1d | +1 | 3 | Ac-Gly-Gly-Val-Gly-Gly-Leu-Gly-Leu-Gly-Lys-amide | 856 | 42 |
| 1e | +1 | 4 | Ac-Gly-Gly-Val-Ala-Gly-Leu-Gly-Leu-Gly-Lys-amide | 870 | 56 |
| 1f | +1 | 4 | Ac-Gly-Gly-Ile-Gly-Gly-Leu-Gly-Leu-Gly-Lys-amide | 870 | 56 |
| 1g | +1 | 5 | Ac-Gly-Gly-Ile-Ala-Gly-Leu-Gly-Leu-Gly-Lys-amide | 884 | 70 |
| 1h | +1 | 7 | Ac-Gly-Gly-Ile-Val-Gly-Leu-Gly-Leu-Gly-Lys-amide | 912 | 98 |
| 1i | +1 | 8 | Ac-Gly-Gly-Ile-Ile-Gly-Leu-Gly-Leu-Gly-Lys-amide | 926 | 112 |
| 2a | +2 | 0 | Gly-Gly-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-amide | 772 | 0 |
| 2b | +2 | 1 | Gly-Gly-Ala-Gly-Gly-Leu-Gly-Leu-Gly-Lys-amide | 786 | 14 |
| 2c | +2 | 2 | Gly-Gly-Ala-Ala-Gly-Leu-Gly-Leu-Gly-Lys-amide | 800 | 28 |
| 2d | +2 | 3 | Gly-Gly-Val-Gly-Gly-Leu-Gly-Leu-Gly-Lys-amide | 814 | 42 |
| 2e | +2 | 4 | Gly-Gly-Val-Ala-Gly-Leu-Gly-Leu-Gly-Lys-amide | 828 | 56 |
| 2f | +2 | 4 | Gly-Gly-Ile-Gly-Gly-Leu-Gly-Leu-Gly-Lys-amide | 828 | 56 |
| 2g | +2 | 5 | Gly-Gly-Ile-Ala-Gly-Leu-Gly-Leu-Gly-Lys-amide | 842 | 70 |
| 2h | +2 | 7 | Gly-Gly-Ile-Val-Gly-Leu-Gly-Leu-Gly-Lys-amide | 870 | 98 |
| 2i | +2 | 8 | Gly-Gly-Ile-Ile-Gly-Leu-Gly-Leu-Gly-Lys-amide | 884 | 112 |
| 3a | +3 | 0 | Gly-Gly-Gly-Gly-Lys-Leu-Gly-Leu-Gly-Lys-amide | 843 | 0 |
| 3b | +3 | 1 | Gly-Gly-Ala-Gly-Lys-Leu-Gly-Leu-Gly-Lys-amide | 857 | 14 |
| 3c | +3 | 2 | Gly-Gly-Ala-Ala-Lys-Leu-Gly-Leu-Gly-Lys-amide | 871 | 28 |
| 3d | +3 | 3 | Gly-Gly-Val-Gly-Lys-Leu-Gly-Leu-Gly-Lys-amide | 885 | 42 |
| 3e | +3 | 4 | Gly-Gly-Val-Ala-Lys-Leu-Gly-Leu-Gly-Lys-amide | 899 | 56 |
| 3f | +3 | 4 | Gly-Gly-Ile-Gly-Lys-Leu-Gly-Leu-Gly-Lys-amide | 899 | 56 |
| 3g | +3 | 5 | Gly-Gly-Ile-Ala-Lys-Leu-Gly-Leu-Gly-Lys-amide | 913 | 70 |
| 3h | +3 | 7 | Gly-Gly-Ile-Val-Lys-Leu-Gly-Leu-Gly-Lys-amide | 941 | 98 |
| 3i | +3 | 8 | Gly-Gly-Ile-Ile-Lys-Leu-Gly-Leu-Gly-Lys-amide | 955 | 112 |
Ac denotes α-acetyl; amide denotes Cα-amide. Standards in the 
and 
groups have a free α-amino group. Variations in composition of the peptide analogs are indicated in bold where the amino acid residues differ from 1a, 2a or 3a.