Table 1.

Primers used for PCR amplification and sequence analysis

Primer	Gene	Virusesa	Sequence 5′ to 3′	Sense	Position	Amplicon size
CCoV1b	M	CCoV, FCoV-like CCoV, FCoV type II	TCCAGATATGTAATGTTCGG	+	6729–6748d	409 bp
CCoV2b			TCTGTTGAGTAATCACCAGCT	−	7138–7118d
CCoV1ac	M	FCoV-like CCoV, FCoV type II	GTGCTTCCTCTTGAAGGTACA	+	6900–6920d	239 bp
CCoV2b			TCTGTTGAGTAATCACCAGCT	−	7138–7118d
UCD1F	S	FCoV-like CCoV	CAGAATGGGAAGAAGTGACG	+	3704–3723e	502 bp
UCD1R			CACACATACCAAGGCCATTTT	−	4185–4205e
V1F	S	FCoV-like CCoV	AAGGACGAGTGCACCGACTAYAAYATHTA	+	2092–2120e	1698 bp
V1R			TGCATACGTGTCATTAACACAA	−	3738–3759e
V2F	S	FCoV-like CCoV	GACGGCTTCTCCTTCAACAAYTGGTTYHT	+	868–896e	1420 bp
V2R			CAGCAGCTTGAGCAGTTAAATC	−	2257–2278e
V3F	S	FCoV-like CCoV	GTTTCTGATGCTATTAGTACTGTTTCC	+	3274–3300e	744 bp
V3R			ACCTTCAGTAAAATCTGGAATTGTG	−	3993–4017e

^aFCoV type I has not been tested.

^bPratelli et al. (1999).

^cPratelli et al. (2002a).

^dPrimers position is referred to the sequence of CCoV strain Insavc-1 (accession: D13096).

^ePrimers position is referred to the sequence of FCoV type I strain UCD1 (accession: AB088222).