Table 1.

Nucleotide sequences of primers and fluorogenic probe

Primer or probea	Sequence (5′ → 3′)b	Tm (°C)c	Locationd within the genome	Size (bp)	GC content (%)
Forward primer	CCCAAGCTGATGACACCTTTG	59.2	2869–2889	21	52.38%
Reverse primer	AATCCAGAGGCTCATCCTGGT	58.6	2945–2965	21	52.38%
Probe	FAM-CGCGTAGAACTGTGACA ACAACGCTGA-TAMRA	68.8	2915–2941	27	51.85%

^aPrimers and probe were designed by using Primer Express V2.0 software.

^bFAM fluorescent reporter dye and with TAMRA the quencher dye.

^cMelting temperature estimated by Primer Express 2.0 primer test document, dyes are not accounted for in the calculations.

^dNucleotide positions are based on GenBank EF641008.