Table 2.
Specificity of real-time RT-PCR for highly virulent Chinese-type PRRSVa
| Genus | Strainb (source, No.) | Original source | Result c |
|---|---|---|---|
| Arterivirus | Highly virulent Chinese-type PRRSV ADCPC 101 | ChongQing, 2006 | + |
| Highly virulent Chinese-type PRRSV ADCPC 123 | ChongQing, 2006 | + | |
| Highly virulent Chinese-type PRRSV ADCPC 131 | ChongQing, 2006 | + | |
| Highly virulent Chinese-type PRRSV ADCPC 145 | ChongQing, 2006 | + | |
| Highly virulent Chinese-type PRRSV ADCPC 152 | ChongQing, 2006 | + | |
| Normal PRRSV ADCPC 1001 | ChongQing, 2001 | − | |
| Normal PRRSV ADCPC 1120 | ChongQing, 2003 | − | |
| Normal PRRSV ADCPC 1321 | ChongQing, 2004 | − | |
| Normal PRRSV ADCPC 1542 | ChongQing, 2005 | − | |
| Normal PRRSV LNCIQ b53 | ShengYang, 2004 | − | |
| Normal PRRSV LNCIQ b55 | ShengYang, 2005 | − | |
| Normal PRRSV SZCIQ 0131 | ShenZheng, 2005 | − | |
| Pestivirus | Classical Swine Fever Virus (CSFV) HNCIQ 3–125 | HuNan, 2004 | − |
| Classical Swine Fever Virus (CSFV) HNCIQ 3–121 | HuNan, 2005 | − | |
| Bovine viral diarrhea virus (BVDV) LNCIQm31 | LiaoNing, 2005 | − | |
| Bovine viral diarrhea virus (BVDV) SHCDC 0260 | Shanghai, 2005 | − | |
| Bovine viral diarrhea virus (BVDV) SHCDC 0317 | Shanghai, 2005 | − | |
| Parvovirus | Porcine parvovirus (PPV) SZCIQ 0138 | ShenZheng, 2004 | − |
| Porcine parvovirus (PPV) SZCIQ 0219 | ShengZheng, 2004 | − | |
| Varicellovirus | Porcine pseudorabies virus (PRV) GZCDC 0564 | Guangdong, 2004 | − |
| Porcine pseudorabies virus (PRV) GZCDC 0958 | Guangdong, 2004 | − | |
| Porcine pseudorabies virus (PRV) ADCPC 1983 | Sichuan, 2005 | − | |
| Coronavirus | Transmissible gastroenteritis virus (TGEV) GZCDC 0874 | Guangzhong, 2006 | − |
| Transmissible gastroenteritis virus (TGEV) GZCDC 0368 | Guangzhong, 2006 | − | |
| Transmissible gastroenteritis virus (TGEV) HNCIQ 7-303 | HuNan, 2005 | − | |
| Circovirus | Porcine circovirus 2 (PCV-2) SZCIQ 0165 | ShenZheng, 2005 | − |
| Porcine circovirus 2 (PCV-2) LNCIQ404 | LiaoNing, 2005 | − | |
| Enterovirus | Swine Vesicular Disease Virus (SVDV) SZCDC 0358 | ShengZheng, 2004 | − |
| Swine Vesicular Disease Virus (SVDV) ADCPC397 | Chongqing, 2005 | − | |
ADCPC, Chongqing Animal Disease Control and Prevention Center, Chongqing, China; LNCIQ, Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian, China; SZCIQ, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China; HNCIQ, Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha, China; SHCDC, Shanghai Center for Disease Control and Prevention, Shanghai, China. GZCDC, Guangzhou Center for Disease Control and Prevention, Guangzhou, China; SZCDC, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
The type of these virus strains had been determined by serological assay or virus isolation, especially highly virulent Chinese-type PRRSV were identified by sequencing.
Results of real-time RT-PCR: +, positive result; −, negative result.