Table 3.

Intra-and inter-assay reproducibility of real-time RT-PCR

Concentration of specimens (TCID50/ml)	Replicate (Cta value)				Ct mean (S.D.b)	%CVc
	1	2	3	4
Intra-assay
105	15.68	15.54	15.41	15.38	15.50 (0.14)	0.9
104	19.01	18.54	18.98	18.72	18.81 (0.23)	1.2
103	21.23	22.43	22.12	21.94	21.93 (0.51)	2.3
102	25.23	25.36	26.02	25.43	25.51 (0.35)	1.4
101	28.56	28.39	28.12	28.40	28.37 (0.17)	0.6
100	31.98	31.77	32.12	31.95	31.96 (0.16)	0.5
Inter-assay
105	14.98	15.72	15.47	15.84	15.50 (0.39)	2.5
104	18.89	18.62	18.63	18.33	18.62 (0.22)	1.2
103	22.31	22.58	22.21	22.02	22.28 (0.22)	1.0
102	25.13	25.46	25.71	24.98	25.32 (0.33)	1.3
101	28.10	28.64	28.01	28.34	28.27 (0.25)	0.9
100	31.25	31.76	32.53	31.89	31.86 (0.54)	1.7

^aCycle threshold value.

^bStandard deviation.

^cCoefficient of variation = S.D./Ct mean.