Table 2.
Details of oligonucleotide probes
| Probe | Size (bp) | TTm (°C) | Binding site | Sequence 5′→3′ |
|---|---|---|---|---|
| IL-2 | 22 | 57.8 | 300→321 | GAG CTA AAT TTA GCA CTT CCT C |
| IL-4 | 24 | 62.8 | 180→203 | CCC CTA AGA ACA CAA GTG ACA AGG |
| IL-6 | 20 | 61.0 | 253→272 | AAC AAC CTG AAT CTT CCG AA |
| IL-10 | 20 | 66.1 | 378→397 | CAC AGG GCA GAA ATC GAT GA |
| IL-12 | 26 | 62.8 | 314→339a | GAA GAG ATC GAT CAT GAA GAT ATC AC |
| IFN-γ | 21 | 60.8 | 244→264 | AAG GAG AAC AAT TTG GCT CTG |
| G3PDH | 20 | 65 | 286→305 | ATC TTC CAG GAG CGA GAT CC |
a
Based on Macaque sequence.
TTm – Theoretical annealing temperature based on 100% identity.