Table 1.

Components of Reverse Transcription Reaction

	Volume per	Volume mix	Final
Reagent	reaction	for N reactions	concentration
5X First strand buffer	4 μl	4 ×N μl	1×
0.1 mM DTT	2 μl	2 ×N μl	0.01 mM
10 mM dNTP	1 μl	N μl	0.5 mM
Random primers (150 ng/μl)	1 μl	N μl	7.5 ng/μl
Reverse transcriptase (200 U/μl)	1 μl	N μl	200 U/reaction
Ribonuclease inhibitor (optional)	1 μl	N μl	40 U/reaction
Total volume of master mix	10 μl	10 ×N μl	–