Table 2.

Components of the PCR

	Volume per	Volume for N	Final
Reagent	reaction	reactionsa	concentration
10X PCR buffer	5 μl	5 ×N μl	1×
MgCl2, 25 mM	5 μl	5 ×N μl	2.5 mM
dNTP, 10 mM	0.5 μl	0.5 ×N μl	0.1 mM
Forward primers, 10 μM	1.25 μl	1.25 ×N μl	0.25 μM
Reverse primers, 10 μM	1.25 μl	1.25 ×N μl	0.25 μM
DNA polymerase (5U/μl)	0.25 μl	0.25 ×N μl	1.25 U/reaction
Water	34.75 μl	34.75 ×N μl	–
Total	48 μl	48 ×N μl	–

^aN = number of 1.5 ml tubes.