Table 4.

Conditions for the Quantitative PCR

Step	Temperature	Time
1. UNG treatment	50°C	2 min
2. Reverse transcription	60°C	40 min
3. Heat inactivation	95°C	5 min
4. Thermal cycling (50 cycles)
Denaturing	95°C	15 sec
Annealing and extension	55°C	1 min