Table 5.

Components of the Quantitative PCR

	Volume per	Volume for N	Final
Reagent	reaction	reactions	Concentration
Water	6.2 μl	6.2 ×N μl	––
5X TaqMan EZ buffer	5 μl	5 ×N μl	1×
Manganese acetate, 25 mM	3 μl	3 ×N μl	3.0 mM
dATP, 10 mM	0.75 μl	0.75 ×N μl	0.3 mM
dUTP, 10 mM	1.5 μl	1.5 ×N μl	0.6 mM
dCTP, 10 mM	0.75 μl	0.75 ×N μl	0.3 mM
dGTP, 10 mM	0.75 μl	0.75 ×N μl	0.3 mM
Forward primers, 50 μM	0.4 μl	0.4 ×N μl	0.8 μM
Reverse primers, 50 μM	0.4 μl	0.4 ×N μl	0.8 μM
Probe, 10 μM	1 μl	1 ×N μl	0.4 μM
rTth DNA polymerase (2.5U/μl)	1 μl	1 ×N μl	2.5 U/reaction
AmpErase UNG (1 U/μl)	0.25 μl	0.25 ×N μl	0.25 U/reaction
Total	21 μl	21 ×N μl	–