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Preparation of baculoviruses for the expression of a GPCR (example for H4R-Gαi2 fusion protein). The gene of interest (in this case a FLAG-tagged hH4R fused to Gαi2 via a His6 linker) is cloned into a pVl1392 transfer vector. The plasmid and the missing part of the baculovirus DNA (BaculoGold™ DNA) are co-transfected into Sf9 cells. The full baculovirus genome is completed in Sf9 cells by homologous recombination. The cells start to release virus particles into the surrounding medium
