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. 2020 Mar 27;11:367. doi: 10.3389/fphar.2020.00367

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Copyright © 2020 Zhu, Li, Li, Zhang, Du, Hao, Li, Cao and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Berberine (BBR) can increase the number of autophagosomes in H9C2 cells subjected to hypoxia/reoxygenation (H/R). Autophagosomes were detected in H9C2 cells pretreated with small interfering RNA against BNIP3 (siBNIP3)/negative control siRNA (siNC) and BBR (50 μM) by transmission electron microscopy (TEM). Scale bar, 200.0 nm (insets) and magnification 120,000×. (B). Quantification of the autophagosomes in different groups is presented as bar graphs. The figures are representative images of three different samples. The data are the means ± SD (n=3). ^*P < 0.05 compared to the control group; ^#P < 0.05 compared to the H/R group; ^&P < 0.05 compared to the BBR+H/R group.