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. 2020 Mar 27;11:367. doi: 10.3389/fphar.2020.00367

Figure 4.

Figure 4

Berberine (BBR) regulates BNIP3-mediated mitochondrial autophagy to attenuate ischemia/reperfusion (I/R) injury in myocardial ischemia-reperfusion injury (MIRI) model rats. After transfection with shBNIP3/shNC, rats were administered BBR orally (300 mg/kg, once a day for 3 consecutive days) or not and then exposed to I/R. (A, B) Western blotting was used to detect the protein expression of LC3, NIX, and P62; GAPDH was used as a control. (C) After I/R, 2,3,5-triphenyl tetrazolium (TTC) staining (10×) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining (400×) was performed on myocardial tissues from each group. (D, E) Statistical analysis of the infarct size and TUNEL index. 1, sham group; 2, I/R group; 3, BBR+I/R group; 4, shNC+BBR+ I/R group; 5,shHIF-1α+BBR+I/R group. The data are the means ± SD (n=3). *P < 0.05 compared to the sham group; #P < 0.05 compared to the I/R group; &P < 0.05 compared to the BBR+I/R group.